SBIO SP BIOC 1206: STRUCTURAL BIOCHEMISTRY (SPECIAL EXAMS)

The course is designed to provide a detailed understanding of the structure of proteins and nucleic acids. Emphasis in this course will be put on the following topics; Amino acids and peptides, their chemistry, structure and classification. The peptide bond, structure and bioactive peptides. The protein structural hierarchy and examples. Isolation of informational bio macromolecules, precipitation of proteins, Chromatography of proteins including mode (ion exchange, size exclusions and affinity) and resolution (HPLC and FPLC), Protein purification tables, Alkali-phenol-lysis for preparation of plasmid DNA, isolation of mRNA by oligo dTcellulose. Sequencing of informational biomacromoleules; Direct sequencing by Edman degradation, Sequencing of proteins by mass spectrometry, Sanger sequencing of DNA, Sequence databases. Solid phase Methods-Merrifield peptide synthesis, synthesis of oligonucleotides, ElectrophoresisNative electrophoresis, SDS PAGE, Isoelectric focusing, 2D SDS PAGE, Blotting methods, Electro elution. Enzymes purification of organalles, membranes and membrane protein from cells; basis of catalysis, Michaelis-Menten equation, Kinetic parameters, Enzyme inhibition. Allosterism and metabolic control.

  • Enrolled students: No students enrolled in this course yet