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This course introduces the principles biophysical techniques used in Biochemistry. Emphasis is put on the following topics: Intrinsic properties of biomolecules. Interaction of light with biomaterials. Principles of the main spectroscopic techniques: UV/VIS, fluorescence and phosphorescence. Instrumentation, measurement modes and formats, practical uses of spectroscopic techniques for the identification and studies of biological objects and processes. Bio-separations. Basics and principle types of bio chromatography, HPLC. Electrophoretic techniques. Detection systems used in bio separations. Visualization and imaging techniques, flow cytometry. Binding, hybridization and cell based assays. Probes and labeling techniques: intrinsic and extrinsic chromo-and fluorophores, fluorescent proteins, radioisotopes, their analytical uses. Principles and

biological use of NMR, ESR and mass-spectrometry techniques